MPTBiotech.com
BioInformatics Services FAQ




What version of NCBI BLAST is BLAST PbH based on?
What does it mean for an application to be Powered by HOWARD (PbH)?
What are the differences between NCBI BLAST and BLAST PbH?
What do you mean by "parallelized"?
What is a Virtual Power Center (VPC)?
What do you mean by BLAST "Power User"?
What happens to my login information?
During what hours can I use the BLAST PbH VPC?
Are there any limitations in usage?
How are jobs prioritized?
What input formats are supported?
What databases are supported?
How often are the databases updated?
What if the database I want to search isn't listed ?
What if I have a proprietary database that I need to search?
What is the maximum job size that I can submit?
Why do I have to wait to start my job?
Does BLAST PbH support batch jobs?
How do I know if my job is still processing versus a problem occurring?
Why do I have to resubmit my job?
What output formats are supported?
How do I access my results?
Can I save my results?
How long are my results saved?
Can other people look at my results?
What settings should I use to optimize my BLAST search?
How do I get help when I have problems running my searches?


What version of NCBI BLAST is BLAST PbH based on?
BLAST PbH is based on NCBI BLAST version 2.2.13. (top)
What does it mean for an application to be Powered by HOWARD (PbH)?
Compute and communication intense applications using PbH take full advantage of the compute resources available. As a result, users get the best price performance for the fastest time to answer at the lowest cost. Powered by HOWARD Software Technologies and Middleware is developed by Massively Parallel Technologies (top)
What are the differences between NCBI BLAST and BLAST PbH?
BLAST PbH combines NCBI BLAST version 2.2.13 with Massively Parallel Technologies patented HOWARD™ software technologies to deliver maximum speedup for BLAST jobs that currently take hours or longer to process. The search process for each individual query is parallelized to take advantage of multiple computers so that your answers are delivered in a fraction of the time compared to NCBI BLAST when processing large jobs. (top)
What do you mean by "parallelized"?
In its simplest form, "parallelized" means that a problem is distributed across multiple computers in order to return results faster. In it's best form, the full compute power of every machine is realized such that if 8 computers are used, results are returned 8x faster than a single computer; 128 computers, 128x faster. Unlike conventional parallelized high performance computing (HPC) applications, PbH applications harness the full power of all computers to deliver maximum performance without the cost and complexity of supercomputers and HPC clusters (i.e. collection of computers working together). (top)
What is a Virtual Power Center (VPC)?
A VPC is where users can run computation and communication intense PbH applications for the best price performance. All that is needed is Internet access and a web browser. VPCs include commercially off the shelf hardware, PbH middleware, and PbH software applications. Please contact sales@massivelyparallel.com if you are interested in acquiring a VPC for your organization or workgroup. (top)
What do you mean by BLAST "Power User"?
BLAST Power Users have very large queries (in terms of number of sequences, size of sequences, and size of databases being searched) and want their answers returned significantly faster. Power Users may or may not have a computer or compute cluster available to run his or her queries. Typical Power User queries can take days or months to complete on traditional cluster systems. For example a user whose BLAST job took 355 hours to complete using standard NCBI tblastn on his system completed in only 3 hours using BLAST PbH tblastn. We look forward to your testimonial after using our system. (top)
What happens to my login information?
Sign-up and login information is for Massively Parallel Technologies internal use only. Massively Parallel Technologies will not distribute or share your information with any third-parties. (top)
During what hours can I use the BLAST PbH VPC?
The BLAST PbH VPC is available twenty-four hours a day, seven days a week. When database updates occur, users will be notified via a status message on the MPTBiotech website (www.mptbiotech.com). (top)
Are there any limitations in usage?
Power Users are limited to a single active log-in and submittal of a single job at a time. Subscriptions for premium services that allow multiple jobs and larger jobs to be submitted are available by contacting support@mptbiotech.com. (top)
How are jobs prioritized?
Jobs will be prioritized on a first come, first serve basis. Multiple jobs submitted will also be prioritized on a first come, first serve basis. (top)
What input formats are supported?
BLAST PbH supports standard FASTA input. In addition, larger queries can be uploaded for processing. (top)
What databases are supported?


  • nr (nucleotide)
    • refseq_rna
    • refseq_genome
    • est
    • est_human
    • est_mouse
    • est_others
    • gss
    • htgs
    • pat
    • pdb
    • month
    • alu_repeats
    • dbsts
    • chromosome
    • wgs
    • env_nt
  • nr (protein)
    • refseq
    • swissprot
    • pat
    • pdb
    • month
    • env_nr
  • unigene (nucleotide)
    • mm.seq.uniq
    • nematostella
    • human genome
(top)
How often are the databases updated?
The default refresh rate is once per month: some databases may be refreshed sooner. To request a different version of a database, please contact support@mptbiotech.com. (top)
What if the database I want to search isn't listed ?
Let us know! Send an e-mail to support@mptbiotech.com listing the name, version, and source of the database and we'll do our best to support your requests. (top)
What if I have a proprietary database that I need to search?
If you have a proprietary database that you want to search using BLAST PbH, contact us at support@mptbiotech.com so we can discuss options to best support your need. (top)
What is the maximum job size that I can submit?
Currently no more than 100 sequences per job can be submitted through the website. Please contact support@mptbiotech.com to arrange processing of larger jobs. (top)
Why do I have to wait to start my job?
There are occasions when the number and size of submitted jobs maximize the use of computer resources. In other words, all of our nodes are working at optimal performance and 100% capacity. A message will displayed indicating your job is in the queue. Once computing resources are available, your job will be run. (top)
Does BLAST PbH support batch jobs?
Yes. Batch processing jobs are supported by sending your request to support@mptbiotech.com. (top)
How do I know if my job is still processing versus a problem occurring?
In most cases, if a problem is encountered processing your job, and error message will be displayed. The message will indicate whether or not you need to resubmit your job. (top)
Why do I have to resubmit my job?
Occasionally a problem may be encountered when processing your job and you will need to resubmit it. If the VPC is offline for maintenance a message will be posted on website. (top)
What output formats are supported?
Currently, only plain text (NCBI format) output is supported. HTML, ASN.1, and tabular output formats will be released soon. (top)
How do I access my results?
Results are stored as files in the user's Results directory. This can be accessed from the user's homepage (after login). The files may be opened directly or downloaded to the user's computer from there. (top)
Can I save my results?
Results are automatically saved in the user's Results directory as mentioned above. Currently the limit on storage space for a user is set to a default of 1GB. If this limit is exceeded, the user will be notified by email and a short period of time will be allowed for the user to rectify the situation. After this grace period, MPT may be forced to remove some data, starting with the oldest results first. (top)
How long are my results saved?
Currently there is no time limit for how long results will be saved as long as you remain under your default 1 GB storage limit. Once your limit is exceeded, you will be notified by email and given a short period of time to offload and/or delete results no longer needed. After this grace period, results wil be removed - starting with the oldest results first - until you are under your 1GB storage limit. (top)
Can other people look at my results?
No. Only you can access your results through your unique login and password. (top)

What settings should I use to optimize my BLAST search?
BLAST PbH accelerates BLAST searches but leaves specific search settings and criteria up to the user. Default settings are based on NCBI BLAST. The NCBI website (www.ncbi.nlm.nih.gov/BLAST/) provides a variety of resources to familiarize users to BLAST. In addition, the book "BLAST", by Ian Korf, Mark Yandell, and Joseph Bedell, is a great reference resource. (top)

How do I get help when I have problems running my searches?
Support@mptbiotech.com can help with the following types of questions and issues:
If you have questions regarding setting-up your searches or specifics of output results, please reference the NCBI website or reference books such as "BLAST", by Ian Korf, Mark Yandell, and Joseph Bedell.
(top)

 

 

Close FAQ